Tuesday, 18 February 2014

Quality check

BOLD effects of interest are small, so temporal stability during functional acquisition is important. In order to accurately measure such small signal changes, an MR system must have intrinsic image time series fluctuation levels much lower than these expected signal changes. Quality checks allow you to assess if your data are worth being analyzed.

Acquisition (artifacts)

  • SNR - signal to noise - single image (contrast to noise, dropout/susceptibility; gray/white matter contrast in structurals)
  • SNR - temporal (e.g., maps of mean / std. deviation across time)
  • ghosting - image wrap-around artifact*

  • image intensity - high enough to avoid information loss? (e.g., >1000)
  • distortions - spatial distortions in acquisition

    Motion artifacts mainly propagate in the phase-encode direction. This is due to movement of the spins between 2 excitations or between phase-encoding and signal reading: in the first case, the spins will not be recorded at the same position between excitations, in the second case, their phase-encoding will not be correct. As a result, the phase-encoding of these voxels is corrupted and this will be responsible for artifacts in the phase-encode direction.
    On the other hand, signal sampling and spatial-encoding in the frequency-encode direction are done so fast that physiological motion will only produce a small amount of spatial blurring in that direction.

  • Spikes - gradient artifacts and bad images *

  • drift - large signal drift over time (look at FFT or timeseries)
  • periodic noise - low-frequency noise artifacts

    When the movements are periodical (cardiac beats, arterial or CSF pulsations, respiration), they can produce ghost images, propagated in the phase-encode direction, even outside the anatomic limits. The intensity of these ghost images becomes more extreme with the intensity of the moving structure and with the amplitude of movement. These ghost images can show up as an increase or decrease of the true image signal.
    The spacing between ghost images varies with the direction of the movement, its amplitude and its periodicity relative to the phase-sampling interval (TR).

  • streaks/striping in images (RF room leaks)

Processing

  • orientation (reconstruction/header problems)
  • skull stripping or segmentation failures (missing brain)
  • coregistration (anatomical to functional overlap)
  • normalization (warping was ok? bad alignment/distortion?)
  • movement estimates (reasonable? too much movement?)

Modeling/statistics

  • colinearity and predictor variance in model
  • mask (which voxels are analyzed?)
  • image registration (consistent across all images in analysis?)
  • contrast scaling (consistent across all images?)
  • indicators of task-correlated artifacts: global shift, large std across contrast
  • outliers (very unusual images)

    Reference: http://wagerlab.colorado.edu/wiki/
    http://www.imaios.com/en/e-Courses/e-MRI/Image-quality-and-artifacts/image-quality

Thursday, 13 February 2014

How to find Resting state networks

GIFT is an application supported by the NIH under grant 1RO1EB000840 to Dr. Vince Calhoun and Dr. Tulay Adali. It is a MATLAB toolbox which implements multiple algorithms for independent component analysis and blind source separation of group (and single subject) functional magnetic resonance imaging data. GIFT works on MATLAB 6.5 and higher.
For any question or comments please contact Vince Calhoun (vcalhoun@unm.edu)or Srinivas Rachakonda ( srachakonda@mrn.org ).

http://mialab.mrn.org/software/gift/index.html

Resting state networks

RSN 1: default mode network, including the posterior cingulate and precuneus, medial prefrontal cortex, dorsal lateral prefrontal cortex and inferior parietal cortex.
RSN 2: dorsal attention network, including the intraparietal sulci, areas at the intersection of precentral and superior frontal sulcus, ventral precentral, and middle frontal gyrus.
RSN 3: visual processing network, including the retinotopic occipital cortex and the temporal-occipital regions.
RSN 4: auditory-phonological network, the superior temporal cortices.
RSN 5: sensory-motor network, including the precentral, postcentral, and medial frontal gyri, the primary sensory-motor cortices, and the supplementary motor area.
RSN 6: self-referential network, including the medial-ventral prefrontal cortex, the pregenual anterior cingulate, the hypothalamus, and the cerebellum.
Mantini et al. (2007)


Resting state pipeline

RS pipeline will pre-process your rsfMRI data so that it can then be used for higher level analysis.

What are the advantages of using the RS pipeline?

The work done through the pipeline will be highly reproducible.

What steps the the RS pipeline currently include?

  1. Convert DICOM data to NIFTI *
  2. Read header for scanning parameters
  3. Slice-timing correction
  4. Motion correction *
  5. Subject space registration *
  6. Normalization to a template *
  7. Detrending
  8. High pass temporal filtering
  9. Regression of WM, GM and motion

http://nipy.org/nipype/

 

Resting state fMRI

Resting state fMRI (rsfMRI or R-fMRI) is a method of functional brain imaging that can be used to evaluate regional interactions that occur when a subject is not performing an explicit task.

 -- Bharat Biswal --

Biswal, B. B. (2012). Resting state fMRI: A personal history. [Review]. Neuroimage, 62(2), p. 938-944

Conference this year:
http://www.martinos.org/brainconnectivity/

Spring school in Ghent:
http://www.da.ugent.be/school2014/#.UxWRoV5kL2c


Frequently asked questions:

What is resting state fMRI? What discerns resting state activity from other types of noise? · Which are the frequency characteristics of current resting state analyses?

How do we process rs-fMRI data? · What are basic analysis strategies of local and interregional activity? · How do we treat physiological noise? · What are the effects of global mean regression? ·

What is connectivity? · What is functional versus structural connectivity? · What is discerned: cross correlation, granger causality, standard seed-based approaches, or (semi) partial correlation? · How can we use rs-fMRI to parcellate the brain? ·

What are brain networks? · What are functional networks of the brain? · How do we use ICA and what is it good for? · What are other network approaches? · How do I analyze graph properties during rest?

What is the default mode network? · What are task positive and task negative networks? · What are functions of the default mode network? · How do we assess within and between network activities?

What is ongoing activity? · How are resting state fluctuations and attention related? ·

What about interindividual variability? · What is reliable, what stable and what changes in rs-fMRI? · What are dynamic properties of connectivity? · Which pharmacological effects do we know? · How is brain development related to its resting state? 

Which clinical aspects can we investigate? · What are robust findings of abnormal resting state behavior in patients? · What is the perfect clinical resting state experiment? · How can we use rs-fMRI in diagnosis and monitoring of patients? · What needs to be solved for multicenter approaches using resting state fMRI?

Brain Connectivity

 Brain Connectivity refers to a pattern of anatomical links ("anatomical connectivity"), of statistical dependencies ("functional connectivity") or of causal interactions ("effective connectivity") between distinct units within a nervous system.

 -- Olaf Sporns --